Composite

Part:BBa_K2355302:Design

Designed by: Cathrine Agnete Larsen   Group: iGEM17_DTU-Denmark   (2017-10-23)


AmilCP-based composite part for insertion of cleavable peptide sequences in RFC[25]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1711
    Illegal AgeI site found at 1725
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1722


Design Notes

The ScAvidin domain can be hard for companies to synthesize due to the many repeats of similar motives in the ScAvidin molecule.



Source

Single chain Avidin with acquired from TU Munich iGEM 2016 (BBa_K2170201) and the RBS is from Berkeley 2008 iGEM team. AmilCP is from Uppsala iGEM 2011 (BBa_K592009).

References